Combinatorial polyketide biosynthesis at higher stage

Combinatorial polyketide biosynthesis at higher stage

Modular polyketide synthases (PKS) of bacteria provide an amazing molecular assembly line for the biosynthesis of complex polyketides. This biosynthesis system has, since its discovery, attracted the attention of scientists and pharmaceutical companies owing to its combinatorial potential. It has provoked the idea of constructing ‘unnatural’ product libraries containing a myriad of compounds wi...

Dissecting complex polyketide biosynthesis

Numerous bioactive natural products are synthesised by modular polyketide synthases. These compounds can be made in high yield by native multienzyme assembly lines. However, formation of analogues by genetically engineered systems is often considerably less efficient. Biochemical studies on intact polyketide synthase proteins have amassed a body of knowledge that is substantial but still incomp...

The stereochemistry of complex polyketide biosynthesis by modular polyketide synthases.

Polyketides are a diverse class of medically important natural products whose biosynthesis is catalysed by polyketide synthases (PKSs), in a fashion highly analogous to fatty acid biosynthesis. In modular PKSs, the polyketide chain is assembled by the successive condensation of activated carboxylic acid-derived units, where chain extension occurs with the intermediates remaining covalently boun...

Diversity-oriented combinatorial biosynthesis of benzenediol lactone scaffolds by subunit shuffling of fungal polyketide synthases.

Combinatorial biosynthesis aspires to exploit the promiscuity of microbial anabolic pathways to engineer the synthesis of new chemical entities. Fungal benzenediol lactone (BDL) polyketides are important pharmacophores with wide-ranging bioactivities, including heat shock response and immune system modulatory effects. Their biosynthesis on a pair of sequentially acting iterative polyketide synt...

Reactive Heterocycles for Examining Polyketide Biosynthesis